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recombinant mouse il 7  (Thermo Fisher)


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    Thermo Fisher recombinant mouse il 7
    (A) Schematic representation of B cell lymphopoiesis and markers that define stages of B cell development. (B, C) Representative contour plots (B) and frequency (C) of bone marrow B220 + CD19 - CD43 + CD93 + cells in C57BL/6 mice with IC injection of PBS or E0771 cells at day 17. (D, E) Representative contour plots (D) and frequency (E) of bone marrow B220 + CD19 + CD43 + CD93 + IgM - pro-B/large pre-B cells in mice described in (B). (F-I) Representative contour plots (F) and frequency of bone marrow B220 + CD19 + CD43 - IgM - IgD - small pre-B cells (G), B220 + CD19 + CD43 - IgM + IgD - immature B cells (H), and B220 + CD19 + CD43 - IgM + IgD + mature B cells (I) in mice described in (B). (J-O) Absolute numbers of B220 + CD19 + B cells (J) and frequency of bone marrow B cell subsets (K-O) in C57BL/6 mice with IC injection of PBS or E0771 cells at day 7. (P, Q) Representative pictures (P) of colony formation unit (CFU) assay of pre-B cells and quantification of CFUs (Q) from bone marrow of mice described in (B). (R) Frequency of bone marrow B cell subsets from bone marrow of mice described in (B) cultured <t>with</t> <t>IL-7</t> for 3 days, relative to day 0. Statistical analysis was performed using unpaired two-tailed Student’s t test (C, E, G-O, Q, R). Data are represented as means ± SEM. Each dot represents a mouse in (C, E, G-O, Q, R). ∗p ≤ 0.05, ∗∗p ≤ 0.01, ∗∗∗p ≤ 0.001, ∗∗∗∗p ≤ 0.0001.
    Recombinant Mouse Il 7, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/recombinant+mouse+il+7/2+MERCAPTOETHANOL/bio_rxiv__64898__2026__04__21__720007-244-28-31
    Average 99 stars, based on 1 article reviews
    recombinant mouse il 7 - by Bioz Stars, 2026-09
    99/100 stars

    Images

    1) Product Images from "Bone marrow B cell collapse promotes bone metastasis in breast cancer"

    Article Title: Bone marrow B cell collapse promotes bone metastasis in breast cancer

    Journal: bioRxiv

    doi: 10.64898/2026.04.21.720007

    (A) Schematic representation of B cell lymphopoiesis and markers that define stages of B cell development. (B, C) Representative contour plots (B) and frequency (C) of bone marrow B220 + CD19 - CD43 + CD93 + cells in C57BL/6 mice with IC injection of PBS or E0771 cells at day 17. (D, E) Representative contour plots (D) and frequency (E) of bone marrow B220 + CD19 + CD43 + CD93 + IgM - pro-B/large pre-B cells in mice described in (B). (F-I) Representative contour plots (F) and frequency of bone marrow B220 + CD19 + CD43 - IgM - IgD - small pre-B cells (G), B220 + CD19 + CD43 - IgM + IgD - immature B cells (H), and B220 + CD19 + CD43 - IgM + IgD + mature B cells (I) in mice described in (B). (J-O) Absolute numbers of B220 + CD19 + B cells (J) and frequency of bone marrow B cell subsets (K-O) in C57BL/6 mice with IC injection of PBS or E0771 cells at day 7. (P, Q) Representative pictures (P) of colony formation unit (CFU) assay of pre-B cells and quantification of CFUs (Q) from bone marrow of mice described in (B). (R) Frequency of bone marrow B cell subsets from bone marrow of mice described in (B) cultured with IL-7 for 3 days, relative to day 0. Statistical analysis was performed using unpaired two-tailed Student’s t test (C, E, G-O, Q, R). Data are represented as means ± SEM. Each dot represents a mouse in (C, E, G-O, Q, R). ∗p ≤ 0.05, ∗∗p ≤ 0.01, ∗∗∗p ≤ 0.001, ∗∗∗∗p ≤ 0.0001.
    Figure Legend Snippet: (A) Schematic representation of B cell lymphopoiesis and markers that define stages of B cell development. (B, C) Representative contour plots (B) and frequency (C) of bone marrow B220 + CD19 - CD43 + CD93 + cells in C57BL/6 mice with IC injection of PBS or E0771 cells at day 17. (D, E) Representative contour plots (D) and frequency (E) of bone marrow B220 + CD19 + CD43 + CD93 + IgM - pro-B/large pre-B cells in mice described in (B). (F-I) Representative contour plots (F) and frequency of bone marrow B220 + CD19 + CD43 - IgM - IgD - small pre-B cells (G), B220 + CD19 + CD43 - IgM + IgD - immature B cells (H), and B220 + CD19 + CD43 - IgM + IgD + mature B cells (I) in mice described in (B). (J-O) Absolute numbers of B220 + CD19 + B cells (J) and frequency of bone marrow B cell subsets (K-O) in C57BL/6 mice with IC injection of PBS or E0771 cells at day 7. (P, Q) Representative pictures (P) of colony formation unit (CFU) assay of pre-B cells and quantification of CFUs (Q) from bone marrow of mice described in (B). (R) Frequency of bone marrow B cell subsets from bone marrow of mice described in (B) cultured with IL-7 for 3 days, relative to day 0. Statistical analysis was performed using unpaired two-tailed Student’s t test (C, E, G-O, Q, R). Data are represented as means ± SEM. Each dot represents a mouse in (C, E, G-O, Q, R). ∗p ≤ 0.05, ∗∗p ≤ 0.01, ∗∗∗p ≤ 0.001, ∗∗∗∗p ≤ 0.0001.

    Techniques Used: Injection, Colony-forming Unit Assay, Cell Culture, Two Tailed Test

    Related Articles

    Cell Culture:

    Article Title: Bone marrow B cell collapse promotes bone metastasis in breast cancer
    Article Snippet: .. 7.5 x 10 5 cells/ml were cultured in IMDM media containing Glutamax (Gibco, 31980030) supplemented with 10% FBS, 1 × P/S and 5μM 2-Mercaptoethanol, and 10 ng/ml of recombinant mouse IL-7 (Invitrogen, RP-8664) in a humidified incubator at 37 °C and 5% CO 2 . ..

    Recombinant:

    Article Title: Bone marrow B cell collapse promotes bone metastasis in breast cancer
    Article Snippet: .. 7.5 x 10 5 cells/ml were cultured in IMDM media containing Glutamax (Gibco, 31980030) supplemented with 10% FBS, 1 × P/S and 5μM 2-Mercaptoethanol, and 10 ng/ml of recombinant mouse IL-7 (Invitrogen, RP-8664) in a humidified incubator at 37 °C and 5% CO 2 . ..



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    (A) Schematic representation of B cell lymphopoiesis and markers that define stages of B cell development. (B, C) Representative contour plots (B) and frequency (C) of bone marrow B220 + CD19 - CD43 + CD93 + cells in C57BL/6 mice with IC injection of PBS or E0771 cells at day 17. (D, E) Representative contour plots (D) and frequency (E) of bone marrow B220 + CD19 + CD43 + CD93 + IgM - pro-B/large pre-B cells in mice described in (B). (F-I) Representative contour plots (F) and frequency of bone marrow B220 + CD19 + CD43 - IgM - IgD - small pre-B cells (G), B220 + CD19 + CD43 - IgM + IgD - immature B cells (H), and B220 + CD19 + CD43 - IgM + IgD + mature B cells (I) in mice described in (B). (J-O) Absolute numbers of B220 + CD19 + B cells (J) and frequency of bone marrow B cell subsets (K-O) in C57BL/6 mice with IC injection of PBS or E0771 cells at day 7. (P, Q) Representative pictures (P) of colony formation unit (CFU) assay of pre-B cells and quantification of CFUs (Q) from bone marrow of mice described in (B). (R) Frequency of bone marrow B cell subsets from bone marrow of mice described in (B) cultured <t>with</t> <t>IL-7</t> for 3 days, relative to day 0. Statistical analysis was performed using unpaired two-tailed Student’s t test (C, E, G-O, Q, R). Data are represented as means ± SEM. Each dot represents a mouse in (C, E, G-O, Q, R). ∗p ≤ 0.05, ∗∗p ≤ 0.01, ∗∗∗p ≤ 0.001, ∗∗∗∗p ≤ 0.0001.
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    Image Search Results


    (A) Schematic representation of B cell lymphopoiesis and markers that define stages of B cell development. (B, C) Representative contour plots (B) and frequency (C) of bone marrow B220 + CD19 - CD43 + CD93 + cells in C57BL/6 mice with IC injection of PBS or E0771 cells at day 17. (D, E) Representative contour plots (D) and frequency (E) of bone marrow B220 + CD19 + CD43 + CD93 + IgM - pro-B/large pre-B cells in mice described in (B). (F-I) Representative contour plots (F) and frequency of bone marrow B220 + CD19 + CD43 - IgM - IgD - small pre-B cells (G), B220 + CD19 + CD43 - IgM + IgD - immature B cells (H), and B220 + CD19 + CD43 - IgM + IgD + mature B cells (I) in mice described in (B). (J-O) Absolute numbers of B220 + CD19 + B cells (J) and frequency of bone marrow B cell subsets (K-O) in C57BL/6 mice with IC injection of PBS or E0771 cells at day 7. (P, Q) Representative pictures (P) of colony formation unit (CFU) assay of pre-B cells and quantification of CFUs (Q) from bone marrow of mice described in (B). (R) Frequency of bone marrow B cell subsets from bone marrow of mice described in (B) cultured with IL-7 for 3 days, relative to day 0. Statistical analysis was performed using unpaired two-tailed Student’s t test (C, E, G-O, Q, R). Data are represented as means ± SEM. Each dot represents a mouse in (C, E, G-O, Q, R). ∗p ≤ 0.05, ∗∗p ≤ 0.01, ∗∗∗p ≤ 0.001, ∗∗∗∗p ≤ 0.0001.

    Journal: bioRxiv

    Article Title: Bone marrow B cell collapse promotes bone metastasis in breast cancer

    doi: 10.64898/2026.04.21.720007

    Figure Lengend Snippet: (A) Schematic representation of B cell lymphopoiesis and markers that define stages of B cell development. (B, C) Representative contour plots (B) and frequency (C) of bone marrow B220 + CD19 - CD43 + CD93 + cells in C57BL/6 mice with IC injection of PBS or E0771 cells at day 17. (D, E) Representative contour plots (D) and frequency (E) of bone marrow B220 + CD19 + CD43 + CD93 + IgM - pro-B/large pre-B cells in mice described in (B). (F-I) Representative contour plots (F) and frequency of bone marrow B220 + CD19 + CD43 - IgM - IgD - small pre-B cells (G), B220 + CD19 + CD43 - IgM + IgD - immature B cells (H), and B220 + CD19 + CD43 - IgM + IgD + mature B cells (I) in mice described in (B). (J-O) Absolute numbers of B220 + CD19 + B cells (J) and frequency of bone marrow B cell subsets (K-O) in C57BL/6 mice with IC injection of PBS or E0771 cells at day 7. (P, Q) Representative pictures (P) of colony formation unit (CFU) assay of pre-B cells and quantification of CFUs (Q) from bone marrow of mice described in (B). (R) Frequency of bone marrow B cell subsets from bone marrow of mice described in (B) cultured with IL-7 for 3 days, relative to day 0. Statistical analysis was performed using unpaired two-tailed Student’s t test (C, E, G-O, Q, R). Data are represented as means ± SEM. Each dot represents a mouse in (C, E, G-O, Q, R). ∗p ≤ 0.05, ∗∗p ≤ 0.01, ∗∗∗p ≤ 0.001, ∗∗∗∗p ≤ 0.0001.

    Article Snippet: 7.5 x 10 5 cells/ml were cultured in IMDM media containing Glutamax (Gibco, 31980030) supplemented with 10% FBS, 1 × P/S and 5μM 2-Mercaptoethanol, and 10 ng/ml of recombinant mouse IL-7 (Invitrogen, RP-8664) in a humidified incubator at 37 °C and 5% CO 2 .

    Techniques: Injection, Colony-forming Unit Assay, Cell Culture, Two Tailed Test